Journal: eLife

Article Title: HA stabilization promotes replication and transmission of swine H1N1 gamma influenza viruses in ferrets

doi: 10.7554/eLife.56236

Figure Lengend Snippet:

Article Snippet: Cell line ( Homo sapiens ) , 293T/17 [HEK 293T/17], Human epithelial kidney expressing SV40 large T antigen , ATCC , ATCC Cat# CRL-11268, RRID: CVCL_1926 , .

Techniques: Expressing, Recombinant, Software, Virus

Journal: Microbiology Spectrum

Article Title: Several Alphaherpesviruses Interact Similarly with the NF-κB Pathway and Suppress NF-κB-Dependent Gene Expression

doi: 10.1128/spectrum.01421-23

Figure Lengend Snippet: Interaction of BoHV-1 with the NF-κB pathway in bovine kidney epithelial cells. (A to C) Western blot analysis of the IκBα protein in BoHV-1-infected (A) and PRV-infected (B) MDBK cells at 8 and 16 hpi (MOI of 10 PFU/cell) or in BoHV-1- or PRV-infected MDBK cells at 8 hpi using an MOI of either 1 PFU/cell or 10 PFU/cell (C). (D and D′) Immunofluorescence detection of the NF-κB p65 subunit in BoHV-1- and PRV-infected MDBK cells at 8 hpi (MOI of 10 PFU/cell). NF-κB p65 is represented in green, and cell nuclei are shown in blue. Arrowheads indicate cells with nuclear p65. The graph in panel D′ shows the mean and standard deviation of three independent replicates. Asterisks indicate statistically significant differences (***, P < 0.001). (E) Light microscopy images showing the cytopathic effect caused by BoHV-1 and PRV infection in MDBK cell monolayers at 8 and 16 hpi (MOI of 10 PFU/cell). (F) Immunofluorescence of viral glycoproteins in MDBK cells infected with BoHV-1 (gC and gD) and PRV (gD) at 8 hpi (MOI of 10 PFU/cell). Viral glycoproteins are shown in green, and the cell nuclei is shown in blue. All assays were independently repeated three times. (G) NF-κB EMSA assessing the in vitro interaction of NF-κB transcription factors with κB sequences using mock-infected MDBK cells, BoHV-1-infected MDBK cells at 16 hpi (MOI of 10 PFU/cell), or PRV-infected MDBK cells at 16 hpi (MOI of 10 PFU/cell). BoHV-1 is indicated as BHV-1 in the figure to save space.

Article Snippet: Bovine kidney epithelial (MDBK) cells (ATCC NBL-1; Bos taurus , cow) were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 4.5g/L d -glucose (DMEM GlutaMAX), 10% inactivated fetal bovine serum (FBS), 1 mM sodium pyruvate, and 50 μg/mL gentamicin.

Techniques: Western Blot, Infection, Immunofluorescence, Standard Deviation, Light Microscopy, In Vitro

Journal: Frontiers in Microbiology

Article Title: Adenovirus Isolated From a Cat Is Related to Human Adenovirus 1

doi: 10.3389/fmicb.2019.01430

Figure Lengend Snippet: Replication of the feline adenovirus in different human ( Homo sapiens ) cell lines.

Article Snippet: Cat ( Felis catus ) , Kidney epithelial , CRFK (ATCC CCL-94) , CPE+ , IFA+, IHC+.

Techniques: Transformation Assay

Journal: Frontiers in Microbiology

Article Title: Adenovirus Isolated From a Cat Is Related to Human Adenovirus 1

doi: 10.3389/fmicb.2019.01430

Figure Lengend Snippet: Replication of the feline adenovirus in different animal cell lines.

Article Snippet: Cat ( Felis catus ) , Kidney epithelial , CRFK (ATCC CCL-94) , CPE+ , IFA+, IHC+.

Techniques: